Tuesday, May 21, 2019

Intracellular recording Lab





Head of lab: Mohammad Shabani; PHD

Intracellular lab was established in 2010 and has continued its activities in the fields of electrophysiology. Using a combination of electrophysiological (whole cell patch clamp) and behavioral studies, we have taken steps to tease out the function of Purkinje cells, Pyramidal motor neurons and CA1 neurons.  In the laboratory our efforts are focused in three areas:

1-      We focus mainly on describing the role and function of Purkinje cells within the cerebellum. Purkinje cells are the sole output of the cerebellar cortex. These spontaneously active cells encode the timing signals required for motor coordination in their rate and pattern of activity. We are also interested in defining the function of motor neurons in neurodegenerative disease in motor cortex.

2-       The goal of our laboratory is to understand how the cerebellum coordinates movement, and how its malfunction in disease causes ataxia and investigating effect of some drug (especially cannabinoids) on neurodegenerative disease such as Parkinson, Tremor and Alzheimer.

3-      We are focusing on some drugs effect on cellular mechanisms of cognition with an emphasis on learning and memory during invivo and invitro in the hippocampus. 

Selected Recent Publications:

1.      Cannabinoid type 1 receptor antagonism ameliorates harmaline-induced essential tremor in rat. Abbassian H, Whalley BJ, Sheibani V, Shabani M. Br J Pharmacol. 2016 Nov;173(22):3196-3207. doi: 10.1111/bph.13581.

2.      Cannabinoid receptor agonism suppresses tremor, cognition disturbances and anxiety-like behaviors in a rat model of essential tremor. Abbassian H, Esmaeili P, Tahamtan M, Aghaei I, Vaziri Z, Sheibani V, Whalley BJ,Shabani M. Physiol Behav. 2016 Oct 1;164(Pt A):314-20. doi: 10.1016/j.physbeh.2016.06.013.

3.      Alterations in the intrinsic electrophysiological properties of Purkinje neurons in a rat model of hepatic encephalopathy: Relative preventing effect of PPARγ agonist. Aghaei I, Hajali V, Dehpour A, Haghani M, Sheibani V, Shabani M. Brain Res Bull. 2016 Mar;121:16-25. doi: 10.1016/j.brainresbull.2015.12.002.

4.      Cannabinoids and Tremor Induced by Motor-related Disorders: Friend or Foe? Arjmand S, Vaziri Z, Behzadi M, Abbassian H, Stephens GJ, Shabani M. Neurotherapeutics. 2015 Oct;12(4):778-87. doi: 10.1007/s13311-015-0367-5. Review.

5.       CB1 cannabinoid receptor activation rescues amyloid β-induced alterations in behaviour and intrinsic electrophysiological properties of rat hippocampal CA1 pyramidal neurones. Haghani M, Shabani M, Javan M, Motamedi F, Janahmadi M. Cell Physiol Biochem. 2012;29(3-4):391-406. doi: 10.1159/000338494.

6.      Maternal exposure to the CB1 cannabinoid agonist WIN 55212-2 produces robust changes in motor function and intrinsic electrophysiological properties of cerebellar Purkinje neurons in rat offspring. Shabani M, Hosseinmardi N, Haghani M, Shaibani V, Janahmadi M. Neuroscience. 2011 Jan 13;172:139-52. doi: 10.1016/j.neuroscience.2010.10.031.